Coliform group bacilli obtained from fecal specimens of 80 healthy children with the age of 10 to 13 years who had no episode of medical treatment with antibiotics during recent 6 months were evaluated for the presence of resistanceto various antibiotics and the demonstration of transmissible rate of Rplasmids.
Among 240 strains of coliform group, 118 strains (46.6%) were resistant to antimicrobial agents such as ampicillin (Ap), kanamycin (Km), streptomycin (Sm), and tetracycline (Tc). In detail, E. coli were 49, Paracolon bacilli 30, Alkalescence-Dispar 26, Providencia 10 and each of Hafnia, Serratia and Citrobacter 1,respectively. Incidence of strains resistant to Tc, Sm, Ap, Cp and Km were 91.5%, 89.0%, 38.8%, 21.20,,-*, and 8.55,/,, respectively. Out of 118 strains, 107 strains were found resistant to two or more of agents.
Thirteen kinds of transmissible- R-plasmids were demonstrated in 46.6% of the resistant strains. Transmissible R-plasmids, however, was not detected in any strain which was resistant only to single agent. Furthermore, the more the resistant agents, the higher the incidence of transmissible resistance. Incidence of the drug resistance strains carrying R-plasmids was 56.7 % in Paracolon bacilli, 53.8% in Alkalescence-Dispar, 42.9% in E. coli and 10%, in Providencia, respectively.
Transmissible rates at 37°C from donors to recipients after 18,24 and 48 hour incubation were 46.6 %, 31.1 %, and 19. 8 %. respeectively.
The present study represents a significant level of infectious drug resistance among the intestinal coliform bacteria of the healthy population.
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